![]() The decrease in flowering time was correlated with the number of mutated BnaSVP alleles. All mutant T 1 lines tested in two environments (summer and winter growing seasons) showed early-flowering phenotypes. Homozygous mutants with loss-of-function alleles and free transgenic elements were generated across the four BnaSVP homologs. The induced mutations in these copies were stably transmitted to subsequent generations. A single construct targeting multiple sites was successfully applied to precisely mutate four copies of BnaSVP. In the present study, BnaSVP, a rapeseed homolog of the Arabidopsis SVP ( Short Vegetative Phase) gene, was characterized and a set of mutants was developed using a CRISPR/Cas9-based gene-editing tool. ![]() ![]() ![]() It is desirable to generate polyploid rapeseed ( Brassica napus L.) germplasm with varying flowering time controlled by a few genes. Manipulation of flowering time to develop cultivars with desired maturity dates is fundamental in plant breeding. ![]()
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